Cholesterol Transporters of the START Domain Protein Family by Barbara J. Clark, Douglas M. Stocco

By Barbara J. Clark, Douglas M. Stocco

Non-vesicular intracellular ldl cholesterol delivery is a vital mechanism for keeping membrane ldl cholesterol homeostasis. contemporary reviews of stories directed at soluble ldl cholesterol shipping proteins point out that aberrant expression of the beginning proteins may perhaps give a contribution to sickness states linked to issues in ldl cholesterol homeostasis. this can be an exhilarating new course within the box and the aim of this publication could be to spotlight the present examine directed at capability roles for the beginning kin in diabetes, melanoma and atherogenesis.

This ebook additionally presents a private and ancient viewpoint of the discovery-to-publication trip that the authors had for his or her specific commence area friend. The objective can be to supply views to graduate scholars, post-doctoral fellows and endocrinology fellows at the study discovery process.

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Additional info for Cholesterol Transporters of the START Domain Protein Family in Health and Disease: START Proteins - Structure and Function

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7 % of the mitochondrial protein (as we later determined). Barbara diligently tried to purify the protein using fast protein liquid chromatography (FPLC). As we had no assay that we could utilize on the fractions collected, she attempted to “find” the protein by running mini 2-D gels on each of the eluted fractions, an unbelievable effort when you think about it. FPLC proved to be unsuccessful and Barbara then went through the arduous process of preparing mitochondrial mitoplasts, solubilizing the protein, running prep gels, cutting out the 28–32 kDa regions of the gels, extracting the proteins from these gels, concentrating the proteins, and running these concentrates on 2-D gels followed by silver staining to visualize the 30 kDa proteins.

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Since the binding site for the repressor complex is not conserved, this may represent a species-(promoter)-specific repression of mouse StAR. In contrast, several repressor proteins have been shown to suppress StAR transcription by both direct and indirect mechanisms in several model systems and promoters. Repressor proteins can bind DNA and recruit corepressor complexes (direct), or block a trans-activator protein from binding DNA (indirect), thereby blocking assembly of a transcription initiation complex.

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