Biosynthesis by E. P. Abraham, G. G. F. Newton (auth.), David Gottlieb, Paul

By E. P. Abraham, G. G. F. Newton (auth.), David Gottlieb, Paul D. Shaw (eds.)

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Studies on the biogenetic origin of carbon atoms 3, 3a and 4 of gliotoxin have shown that these arise from serine or glycine plus a one-carbon unit (WINSTEAD and SUHADOLNIK, 1960). The incorporation of glycine probably proceeds via serine. The biogenetic origin of the N-methyl group of gliotoxin was shown to arise from methionine-CH3-14C, serine-3-14C and glycine-2-14C (WINSTEAD and SUHA- Gliotoxin 31 DOLNIK, 1960). 3 % of the carbon-14 from these three compounds respectively, were found in the N-methyl group of gliotoxin.

Ind. Eng. Chern. 42, 1775 (1950). 42 D. GOTTLIEB: Chloramphenicol REBSTOCK, M. , H. M. CROOKS, J. CONTROULIS, and Q. R BARTZ: Chloramphenicol (chloromycetin). IV. Chemical studies. J. Am. Chern. Soc. 71, 2458 (1949). RINEHART, K. , W. P. O'NEAL, and D. GOTTLIEB: Research currently underway 1966. SMITH, CH. : Effect of halogens on the chloramphenicol fermentation. J. Bacteriol. 75,577 (1958). , D. A. JOSLYN, O. M. GRUHZIT, 1. W. McLEAN, M. A. PENNER, and J. EHRLICH: Chloromycetin: biological studies.

Synthesis /00 J of xanthocillin starts approximately 4 9 days II s 7 days after inocculation and increases as Fig. 1. Concentration of xanthociIlin in cultures of Penicillium notatum shown in Fig. 1. Obviously there is some relation between the observation of autoWestling lysis in the mycelium and the increase in the formation of xanthocillin. On approximately the 9th day the concentration of xanthocillin reaches its climax. Yields above 1 mg antibiotic per milliliter of fermentation broth are possible.

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